Genetic manipulation of dendritic cells for immune regulation

Description of projects available to graduate students:
The broad objective of this project is to treat autoimmune hosts with autologous dendritic cells engineered in a number of ways targeting complementary internal and external signaling pathways. The laboratory is currently studying these modalities in mouse models of type 1 diabetes and atopy. In addition, the laboratory is also studying mechanisms of action of such therapy and of interest are regulatory T- and B-cells

Techniques graduate student will learn:
The techniques to be employed include DNA/RNA expression studies using real-time PCR, antisense DNA technology, cell culture, in vivo administration of dendritic cells, flow cytometry, in vitro immunoassays (MLR, cytokine measurements).

The laboratory has readily-available access to state-of-the art flow cytometry facilities, real-time PCR, and proteomics/mass spectrometry facilities. Technical assistance is friendly and readily available.