Mechanism of Treg induction by DC

Description of projects available to graduate students:
DC presenting low dose antigen induce expansion of Treg in vitro and in vivo, This is good for diabetes but not good for cancer vaccines. We are exploring the mechanisms by which DC induce Treg in the context of type 1 diabetes and cancer vaccines

Techniques graduate student will learn:
DC and T cell culture and purification. Analysis of cytokine production by ELISA, ICC and ELISPOT, Analysis of signaling cascades in T cells using flow cytometry and Western blot