Mechanism and regulation of site-specific recombinationDate Added: 5/30/2006 2:19:00 PM
Last Updated: 5/30/2006 2:19:00 PM
Description of projects available to graduate students:
Mycobacteriophage Bxb1 integrates its DNA into the host genome using a novel serine-integrase enzyme. We have isolated a collection of integrase mutants that are unable to perform recombination and using these to dissect the mechanisms of recombination. We not only have a procient in vitro reocmbination system available to us, but also have a number of in vitro assays that enable us to examine different part of teh pathway, including protein dimerization, DNA binding, synapsis, initiation of strand exchange, strand rotation, and rejoining. A new set of suicide substrates are particuarly informative and in this project you will take a selected set of mutants and characterize their ability to bind and synapse these substrates.
Techniques graduate student will learn:
Biochemistry, DNA binding, gel electrophoresis etc.
Molecular Virology And Microbiology
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