Structure-Function studies of novel IL-17R family membersDate Added: 5/25/2011 5:17:00 PM
Last Updated: 5/15/2013 11:47:00 AM
Description of projects available to graduate students:
The IL-17 receptor is the founding member of a unique subclass of cytokine receptors (Gaffen, Nature Reviews Immunology, 2009). One approach that the Gaffen lab takes to understanding the IL-17R family is to perform molecular deletion and mutation analyses of IL-17R subunits to determine subdomains important for signal transduction. In this project, you will participate in studies of poorly understood IL-17R subunits (e.g., IL-17RE) to define downstream signaling events and identify proteins that associate with specific regions of the receptor. References: Maitra et al. PNAS 2007; Onishi et al J Biol Chem 2010; Ho et al. J Immunol 2010; Gaffen Nature Reviews Immunol 2009
Techniques graduate student will learn:
cell culture, recombinant DNA engineering, transfection and expression of mutant receptors, flow cytometry, western blotting, ELISA, real time PCR, immunoprecipitation, microscopy, and other techniques as required for the specific project.
Molecular Genetics and Developmental Biology
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