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Research
Interests |
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- We are interested in understanding the regulation of heavy chain immunoglobulin (Ig) RNA processing and the development of immunological memory during B-cell development. Alterations in the homeostatic regulation of Ig secretion may result in immunodeficiency -like states while overproduction can occur in diseases like amyloidosis or myeloma. Understanding the basic mechanisms of secretory mRNA production may lead to insights for control and disease amelioration.
- We are also studying the regulation of polyadenylation in a variety of cells including macrophages after LPS stimulation. Changes in the polyadenylation capacity of the cells represents a novel form of gene regulation linking transcription and RNA processing.
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Selected
Publications |
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- Shell SA, Martincic K, Tran J, Milcarek C. Increased phosphorylation of the carboxyl terminal domain of RNA polymerase II and loading of polyadenylation and co-transcriptional factors contribute to the regulation of Ig heavy chain mRNA plasma cells. J Immunol 179(11):7663-73. 2007.
- Borghesi L, Milcarek, C. Innate versus adaptive immunity: a paradigm past its prime? In Cancer Research 67: 3989-3993. 2007.
- Borghesi L, Milcarek C. From B cell to plasma cell: regulation of V(D)J recombination and antibody secretion. Immunological Research 36(1-3):27-32. 2006.
- Alkan SA, Martincic K, Milcarek C. The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J 393:361-371. 2006.
- Shell S, Hesse C, Morris S, Milcarek C. Elevated levels of the 64-kDa cleavage stimulatory factor (CstF-64) in LPS-stimulated macrophages influence gene expression and induce alternative poly(A) site selection. J Biol Chem 280(48):39950-61. 2005.
- Milcarek C, Martincic K, Chung-Ganster L-H, Lutz CS. The snRNP-associated U1A levels change following IL-6 stimulation of human B-cells. Molecular Immunology 39:809-814. 2003.
- Arhin GK, Boots M, Bagga PS, Milcarek C, Wilusz J. Downstream Sequence Elements with Different Affinities for the hnRNP H/H' Protein Influence the Processing Efficiency of Mammalian Polyadenylation Signals. Nucleic Acids Research 30:1842-1850. 2002.
- Edwalds-Gilbert G, Veraldi KL, Milcarek C. Alternative poly(A) site selection in complex transcription units; a means to an end? Nucleic Acids Research 25:2547-2561. 1997.
Complete Publication Listing
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Other
Links |
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University of Pittsburgh |
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Office:
E1054 BST |
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Lab:
E1011, E1008 BST |
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Phone:
412.648.9098 |
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Fax:412.383.8096 |
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milcarek@pitt.edu |
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Academic
Affiliations |
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- Professor, Department of Immunology, University of Pittsburgh School of Medicine
- Member, Graduate Programs in Immunology and Biochemistry and Molecular Genetics
- Chair, Committee on the Status of Women, American Association of Immunologists (2007-2009)
- Member, Committee on the Status of Women, American Association of Immunologists (2006-2009)
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Education |
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- B.S. - Duquesne University
- Ph.D. - Johns Hopkins University
- Postdoc - Massachusetts Institute of Technology
Honors
- Distinguished Mentor (1999) - Survival Skills and Ethics Program, University of Pittsburgh
- Woman of Distinction (2000) - Girl Scouts of Southwestern PA (Trillium Council)
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Grant
Support |
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- Title: Polyadenylation factors in B-cells, lymphoma & Myeloma
Agency: NIH
Role: P.I.
Funding Period: 2003-2009
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Lab
Personnel |
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Research Specialist
Kathleen Martincic |
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