Research Interests The KSHV Lab at the Hillman Cancer Center is jointly run by Dr. Patrick Moore, MGB, and Dr. Yuan Chang, Pathology. There are two broad interests for our group. First, KSHV and its biology: We are actively examining the ability of KSHV to induce cell transformation and malignancy through focused studies on likely viral oncogenes. This includes functional studies of LANA1 and LANA2, vIL-6 and vIRF. The overall goal of these studies is to understand how and why some viruses target specific tumor suppressor pathways. Studies from our group suggest that intracellular innate immune signaling pathways use tumor suppressor control mechanisms to inhibit viral replication. Rotations in this area involve routine molecular biology techniques including cloning, immunoblotting, immunoprecipitation assays, cell culture and immunohistochemistry as well as specific functional assays including electromobility shift assays, luciferase reporter assays, phosphorylation assays and cell transformation assays. New pathogen discovery: In 1993, we identified KSHV using representational difference analysis from a Kaposi sarcoma lesion. We are continuing to search for new and novel pathogens by molecular techniques and taking advantage of the extensive tissue bank facilities available at the University of Pittsburgh.   Selected Publications Ennas MG, Moore PS, Zucca M, Angelucci E, Cabras MG, Melis M, Gabbas A, Serpe R, Madeddu C, Scarpa A, Cocco P. Interleukin-1B (IL1B) and interleukin-6 (IL6) gene polymorphisms are associated with risk of chronic lymphocytic leukaemia. Hematol Oncol. 2008 Jun;26(2):98-103. Feng H, Taylor JL, Benos PV, Newton R, Waddell K, Lucas SB, Chang Y, Moore PS. Human transcriptome subtraction by using short sequence tags to search for tumor viruses in conjunctival carcinoma.J Virol. 2007 Oct;81(20):11332-40. Kwun HJ, da Silva SR, Shah IM, Blake N, Moore PS, Chang Y. Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen 1 mimics Epstein-Barr virus EBNA1 immune evasion through central repeat domain effects on protein processing. J Virol. 2007 Aug;81(15):8225-35. Moore PS, Chang Y, Jaffe HW. Transmission of human herpesvirus 8 by blood transfusion. N Engl J Med. 2007 Jan 4;356(1):88; author reply 89. No abstract available. Laney AS, Peters JS, Manzi SM, Kingsley LA, Chang Y, Moore PS. Use of a multiantigen detection algorithm for diagnosis of Kaposi's sarcoma-associated herpesvirus infection.J Clin Microbiol. 2006 Oct;44(10):3734-41. Complete Publication Listing     Other Links MMG Faculty Webpage http://pathology.cpmc.columbia.edu/C&M/CM_LAB.html Molecular Virology Program University of Pittsburgh

Patrick S Moore, MD MPH Office:  Research Pavillion Suite 1.8, Hillman Cancer Center, 5117 Centre Ave, PGH, PA Lab:Research Pavillion Suite 1.8, Hillman Phone:412-623-7718 Fax: 412-623-7715 psm9@pitt.edu   Academic Affiliations Professor, Department of Microbiology and Molecular Genetics University of Pittsburgh School of Medicine Director, Molecular Virology Program University of Pittsburgh Cancer Institute Member, Biochemistry and Molecular Genetics Graduate Program University of Pittsburgh School of Medicine   Education 1977 B.S. Biology/Chemistry Westminster College Salt Lake City, UT 1980 M.S. Biophysical Chemistry Stanford University Stanford, CA 1985 M.D./MPhil Medicine/Cell Biology University of Utah Salt Lake City, UT 1990 M.P.H. Epidemiology University of California Berkeley, CA   Lab Personnel Administrator: Chrissie Usher Phone: 412/623-7721