Research Interests The KSHV Lab at the Hillman Cancer Center is jointly run by Dr. Patrick Moore, MGB, and Dr. Yuan Chang, Pathology. There are two broad interests for our group. First, KSHV and its biology: We are actively examining the ability of KSHV to induce cell transformation and malignancy through focused studies on likely viral oncogenes. This includes functional studies of LANA1 and LANA2, vIL-6 and vIRF. The overall goal of these studies is to understand how and why some viruses target specific tumor suppressor pathways. Studies from our group suggest that intracellular innate immune signaling pathways use tumor suppressor control mechanisms to inhibit viral replication. Rotations in this area involve routine molecular biology techniques including cloning, immunoblotting, immunoprecipitation assays, cell culture and immunohistochemistry as well as specific functional assays including electromobility shift assays, luciferase reporter assays, phosphorylation assays and cell transformation assays. New pathogen discovery: In 1993, we identified KSHV using representational difference analysis from a Kaposi sarcoma lesion. We are continuing to search for new and novel pathogens by molecular techniques and taking advantage of the extensive tissue bank facilities available at the University of Pittsburgh.   Selected Publications Feng H, Taylor JL, Benos PV, Newton R, Waddell K, Lucas SB, Chang Y, Moore PS. Human transcriptome subtraction using short sequence tags to search for tumor viruses in conjunctival carcinoma. J Virol. 2007 Aug 8; Kwun HJ, da Silva SR, Shah IM, Blake N, Moore PS, Chang Y. Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen 1 mimics Epstein-Barr virus EBNA1 immune evasion through central repeat domain effects on protein processing. J Virol. 2007 Aug;81(15):8225-35. Epub 2007 May 23. Moore PS, Chang Y, Jaffe HW. Transmission of human herpesvirus 8 by blood transfusion. N Engl J Med. 2007 Jan 4;356(1):88; author reply 89. No abstract available. Laney AS, Peters JS, Manzi SM, Kingsley LA, Chang Y, Moore PS. Use of a multiantigen detection algorithm for diagnosis of Kaposi's sarcoma-associated herpesvirus infection. J Clin Microbiol. 2006 Oct;44(10):3734-41. Jacobs SA, Vidnovic N, Patel H, Soma LA, Chang Y, Bass N, Swerdlow SH. Durable remission of HIV-negative, Kaposi's sarcoma herpes virus-associated multicentric Castleman disease in patient with rheumatoid arthritis treated with methotrexate. Clin Rheumatol. 2007 Jul;26(7):1148-50. Epub 2006 Jul 6.   Grant Support NIH: Enhanced cell-mediated immunogenicity of KSHV LANA1 protein. Co-Investigator NIH: Innovative technologies applied to the discovery of human tumor viruses. Co-Investigator NIH: Evolution, biogenesis, and function of microTNA's. Co-Investigator   Other Links University of Pittsburgh

Yuan Chang, Professor, Basic Research Division Office:  Suite 1.8 Hillman Cancer Center Lab: Phone: 412/623-7716 Fax: 412/623-7715 yc70@pitt.edu   Academic Affiliations Professor Pathology University of Pittsburgh Cancer Institute 2002 - present   Education 1981 - B.S. Biological Sciences Stanford University Stanford, CA 1987 - M.D. Medicine University of Utah Salt Lake City, Utah   Lab Personnel Administrator: Chrissie Usher